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- Title
NanoSIMS analysis of arsenic and selenium in cereal grain.
- Authors
Moore, Katie L.; Schröder, Markus; Lombi, Enzo; Fang-Jie Zhao; McGrath, Steve P.; Hawkesford, Malcolm J.; Shewry, Peter R.; Grovenor, Chris R. M.
- Abstract
•Cereals are an important source of selenium (Se) to humans and many people have inadequate intakes of this essential trace element. Conversely, arsenic (As) is toxic and may accumulate in rice grain at levels that pose a health risk. Knowledge of the localization of selenium and arsenic within the cereal grain will aid understanding of their deposition patterns and the impact of processes such as milling. •High-resolution secondary ion mass spectrometry (NanoSIMS) was used to determine the localization of Se in wheat ( Triticum aestivum) and As in rice ( Oryza sativa). Combined synchrotron X-ray fluorescence (S-XRF) and NanoSIMS analysis utilized the strengths of both techniques. •Selenium was concentrated in the protein surrounding the starch granules in the starchy endosperm cells and more homogeneously distributed in the aleurone cells but with Se-rich hotspots. Arsenic was concentrated in the subaleurone endosperm cells in association with the protein matrix rather than in the aleurone cells. NanoSIMS indicated that the high intensity of As identified in the S-XRF image was localized in micron-sized hotspots near the ovular vascular trace and nucellar projection. •This is the first study showing subcellular localization in grain samples containing parts per million concentrations of Se and As. There is good quantitative agreement between NanoSIMS and S-XRF.
- Subjects
GRAIN; RICE; SECONDARY ion mass spectrometry; X-ray spectroscopy; SELENIUM in agriculture; SOIL composition; ARSENIC; TRACE elements; PLANT physiology; PLANT cells &; tissues
- Publication
New Phytologist, 2010, Vol 185, Issue 2, p434
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/j.1469-8137.2009.03071.x