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- Title
Premature apoptosis of keratinocytes and the dysregulation of keratinization in porokeratosis.
- Authors
Shen, C-S.; Tabata, K.; Matsuki, M.; Goto, T.; Yokochi, T.; Yamanishi, K.
- Abstract
Summary Background Porokeratosis is a dyskeratotic disorder of the skin characterized by cornoid lamella with parakeratosis, hyperkeratosis and loss of granular layers. The pathogenesis of porokeratosis and the mechanism(s) of its abnormal keratinization are still unknown. Objective To elucidate the mechanism(s) of abnormal keratinization that leads to the formation of cornoid lamellae in porokeratosis. Methods Apoptosis of keratinocytes was assessed in the skin of seven patients by an in situ apoptosis assay based on the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling (TUNEL) reaction. Patterns of loricrin and involucrin expression were examined by immunohistochemistry. Results TUNEL-positive keratinocytes were observed in the epidermis underlying the cornoid lamella in all cases examined. Furthermore, loricrin expression was interrupted there, in contrast to involucrin, which was expressed diffusely in the lesional epidermis. Conclusions These results suggest that an abnormal early keratinocyte apoptosis accompanied by dysregulation of terminal differentiation of those cells may be involved in the pathogenesis of porokeratosis.
- Subjects
SKIN diseases; KERATINOCYTES; APOPTOSIS; KERATINIZATION
- Publication
British Journal of Dermatology, 2002, Vol 147, Issue 3, p498
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1046/j.1365-2133.2002.04853.x