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- Title
Hydrophilic C terminus of Salicornia europaea vacuolar Na/H antiporter is necessary for its function.
- Authors
WU, GUANGXIA; WANG, GANG; JI, JING; TIAN, XIAOWEI; GAO, HAILING; ZHAO, QING; LI, JING; WANG, YURONG
- Abstract
Plant vacuolar Na/H antiporters play important roles in cellular ion homeostasis, vacuolar pH regulation and sequestration of Na ions into the vacuole. Previous research showed that hydrophilic C-terminal region of Arabidopsis AtNHX1 negatively regulates the Na/H transporting activity. In this study, we truncated the hydrophilic C terminus of a vacuolar Na/H antiporter gene from Salicornia europaea ( SeNHX1) to generate its derivative, SeNHX1- ΔC. Expression of SeNHX1 and SeNHX1- ΔC in yeast mutant showed that SeNHX1 significantly improved the tolerance to NaCl; however, the expression of SeNHX1- ΔC enormously decreased the tolerance to NaCl. Overall, these results suggest that the hydrophilic C-terminal region of SeNHX1 is required for Na/H exchanging activity of SeNHX1.
- Subjects
HYDROPHILIC compounds; SALICORNIA; SODIUM-hydrogen antiporter; HOMEOSTASIS; ARABIDOPSIS; ION transport (Biology); SODIUM-hydrogen exchange
- Publication
Journal of Genetics, 2014, Vol 93, Issue 2, p425
- ISSN
0022-1333
- Publication type
Article
- DOI
10.1007/s12041-014-0396-6