We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Complete cDNA sequence and genomic organization of a human pancreas-specific gene homologous to Caenorhabditis elegans sel-1.
- Authors
Harada, Yosuke; Ozaki, Kouichi; Suzuki, Mikio; Fujiwara, Tsutomu; Takahashi, Ei-ichi; Nakamura, Yusuke; Tanigami, A.
- Abstract
Abstract We have isolated the complete cDNA of a human SEL-1L gene, termed TSA305, that is abundantly expressed only in the pancreas. The cDNA contained an open reading frame of 2382 nucleotides, encoding a deduced protein of 794 amino acids whose predicted sequence showed 46% identity and 64% similarity with SEL-1 of Caenorhabditis elegans. SEL-1 is thought to be a negative regulator of the NOTCH, LIN-12, and GLP-1 receptors, which are required for differentiation and maturation of cells as well as cell-cell interactions during development in C. elegans. The degree of homology among these proteins suggests that the 7SA305 gene product may be a member of the SEL-1 family and therefore involved in downregulation of mammalian Notch signaling. Direct sequencing revealed at least 20 coding exons in TSA305. We localized the gene to chromosome bands 14q24.3-q31 by radiation hybrid (RH) mapping and fluorescence in situ hybridization (FISH). The IDDM11 locus has been mapped in this region, and TSA305 may represent a candidate gene for predisposition in some families whose insulin-dependent diabetes is not linked to the HLA locus.
- Subjects
ANTISENSE DNA; NUCLEOTIDE sequence; CAENORHABDITIS elegans; GENETICS
- Publication
Journal of Human Genetics, 1999, Vol 44, Issue 5, p330
- ISSN
1434-5161
- Publication type
Article
- DOI
10.1007/s100380050171