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- Title
The Diversity of Ribonuclease P: Protein and RNA Catalysts with Analogous Biological Functions.
- Authors
Klemm, Bradley P.; Wu, Nancy; Yu Chen; Xin Liu; Kaitany, Kipchumba J.; Howard, Michael J.; Fierke, Carol A.
- Abstract
Ribonuclease P (RNase P) is an essential endonuclease responsible for catalyzing 5' end maturation in precursor transfer RNAs. Since its discovery in the 1970s, RNase P enzymes have been identified and studied throughout the three domains of life. Interestingly, RNase P is either RNA-based, with a catalytic RNA subunit, or a protein-only (PRORP) enzyme with differential evolutionary distribution. The available structural data, including the active site data, provides insight into catalysis and substrate recognition. The hydrolytic and kinetic mechanisms of the two forms of RNase P enzymes are similar, yet features unique to the RNA-based and PRORP enzymes are consistent with different evolutionary origins. The various RNase P enzymes, in addition to their primary role in tRNA 5' maturation, catalyze cleavage of a variety of alternative substrates, indicating a diversification of RNase P function in vivo. The review concludes with a discussion of recent advances and interesting research directions in the field.
- Subjects
RIBONUCLEASE P; TRANSFER RNA; ENDONUCLEASES; BINDING sites; TECHNOLOGICAL innovations
- Publication
Biomolecules (2218-273X), 2016, Vol 6, Issue 2, p27
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom6020027