We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Viral Metagenomic Analysis of Cerebrospinal Fluid from Patients with Acute Central Nervous System Infections of Unknown Origin, Vietnam.
- Authors
Nguyen To Anh; Le Nguyen Truc Nhu; Nguyen Thi Thu Hong; Tran My Phuc; Pham Thi Thanh Tam; Dang Thao Huong; Tran Tuan Anh; Xutao Deng; Ho Dang Trung Nghia; Tran Thua Nguyen; Nguyen Van Hung; Nguyen Dac Thuan; Pham Thi Hong Phuong; Nguyen Van Vinh Chau; Baker, Stephen; Delwart, Eric; Thwaites, Guy; Le Van Tan; Anh, Nguyen To; Nhu, Le Nguyen Truc
- Abstract
Central nervous system (CNS) infection is a serious neurologic condition, although the etiology remains unknown in >50% of patients. We used metagenomic next-generation sequencing to detect viruses in 204 cerebrospinal fluid (CSF) samples from patients with acute CNS infection who were enrolled from Vietnam hospitals during 2012-2016. We detected 8 viral species in 107/204 (52.4%) of CSF samples. After virus-specific PCR confirmation, the detection rate was lowered to 30/204 (14.7%). Enteroviruses were the most common viruses detected (n = 23), followed by hepatitis B virus (3), HIV (2), molluscum contagiosum virus (1), and gemycircularvirus (1). Analysis of enterovirus sequences revealed the predominance of echovirus 30 (9). Phylogenetically, the echovirus 30 strains belonged to genogroup V and VIIb. Our results expanded knowledge about the clinical burden of enterovirus in Vietnam and underscore the challenges of identifying a plausible viral pathogen in CSF of patients with CNS infections.
- Publication
Emerging Infectious Diseases, 2021, Vol 27, Issue 1, p205
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2701.202723