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- Title
Diagnostic Assays for Crimean-Congo Hemorrhagic Fever.
- Authors
Vanhomwegen, Jessica; Alves, Maria João; Županc, Tatjana Avšič; Bino, Silvia; Chinikar, Sadegh; Karlberg, Helen; Korukluoğlu, Gülay; Korva, Miša; Mardani, Masoud; Mirazimi, Ali; Mousavi, Mehrdad; Papa, Anna; Saksida, Ana; Sharifi-Mood, Batool; Sidira, Persofoni; Tsergouli, Katerina; Wölfel, Roman; Zeller, Hervé; Dubois, Philippe
- Abstract
Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.
- Subjects
HEMORRHAGIC fever; TICK-borne diseases; IMMUNOGLOBULIN M; REVERSE transcriptase; INFECTION; IMMUNOFLUORESCENCE
- Publication
Emerging Infectious Diseases, 2012, Vol 18, Issue 12, p1958
- ISSN
1080-6040
- Publication type
Article
- DOI
10.3201/eid1812.120710