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- Title
Production of acidic xylooligosaccharides from methylglucuronoarabinoxylans by Bacillus subtilis strain MR44.
- Authors
Wei, Lusha; Rhee, Mun Su; Preston, James F; Chen, Hui
- Abstract
BACKGROUND Bacillus subtilis 168 secretes endoxylanases Xyn11A and Xyn30C and arabinofuranohydrolase Axh43 that process xylans in hemicellulose fractions. Engineered strain MR44 with a deletion in the xynA gene encoding Xyn11A, converts hardwood methylglucuronoxylans ( MeGXn) of dicots to defined acidic xylooligosaccharides (U- XOS) with a single methylglucuronate linked to xylooligosaccharides with an average ratio of one methylglucuronate to 6-7 xyloses as value-added products. The MR44 strain also secretes Axh43 that catalyses release of arabinose from methylglucuronoarabinoxylans ( MeGAXn) of monocots. RESULTS MR44 strain efficiently processes sorghum MeGAXn with 493 mg g−1 yields of purified U- XOS. 1H-NMR analysis of U- XOS purified by anion exchange chromatography structurally defined the U- XOS with an average degree of polymerization of xylose units ( DP) of 11-12 and a single 4-O-methyl-glucuronic acid α-1,2-linked on average to one of 11-12 xylose residues each penultimate to the reducing terminal xylose. CONCLUSION This study demonstrates the ability of Bacillus subtilis strains to process MeGAXn for production of U- XOS from monocots, including energy crops and agricultural residues. The U- XOS with an average DP of 11-12 from sweet sorghum MeGAXn compared with 6-7 from sweetgum MeGXn extends their applications as biologicals and for production of pentosan polysulfates with applications in human and veterinary medicine. © 2015 Society of Chemical Industry
- Subjects
OLIGOSACCHARIDES; ARABINOXYLANS; BACILLUS subtilis; XYLANASES; HYDROLASES; XYLANS; HEMICELLULOSE; VETERINARY medicine
- Publication
Journal of Chemical Technology & Biotechnology, 2016, Vol 91, Issue 7, p2056
- ISSN
0268-2575
- Publication type
Article
- DOI
10.1002/jctb.4800