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- Title
Cleavage of Translation Factor NAT1/DAP5/p97 During CoxsackievirusB3 Infection by a Cellular Serine-like Protease.
- Authors
Kim, H.; Hemida, M.; Ye, X.; Zhang, M.; Yang, D. C.
- Abstract
NAT1, also named death-associated protein 5 (DAP5) and p97, is one of the host translation initiation factors. Although there have been ongoing debates about its role, recent studies have suggested that it has a role in up-regulating cap-independent translation, thus favoring translation of CVB3 RNA. In this project, we previously identified that NAT1 protein is cleaved into two smaller fragments by a serine like protease using protease inhibitor assay. We also found that the protease is neither of the well known viral proteases 2A or 3C, nor the caspase-3. Our goal is to identify the cellular protease responsible for this cleavage as well as the specific cleavage site through site-directed mutagenesis. Our working hypothesis is that a serine like cellular protease cleaves NAT1 at a region between the 380th and 435th amino acids during CVB3 infection, producing two peptides of 44 kDa and 53 kDa. We have successfully created a mutation with a 26-amino acid deletion and the transfection, infection and protein analyses are in the process currently.
- Subjects
COXSACKIEVIRUSES; SCISSION (Chemistry); PROTEINS; COXSACKIEVIRUS diseases; GENE transfection
- Publication
UBC Medical Journal, 2011, Vol 2, Issue 2, p39
- ISSN
1920-7425
- Publication type
Abstract