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- Title
Molecular characterization of Vibrio parahaemolyticus isolated from shellfish and their harvesting water from Suez Canal area, Egypt.
- Authors
Youssef, A. I.; Farag, A. L.; Helal, I. M.
- Abstract
Vibrio parahaemolyticus is a leading cause of seafood-derived food poisoning throughout the world. The main objectives of this study were to determine the prevalence of contamination of shellfish by V. parahaemolyticus in Suez Canal area and to assess its molecular characteristics. The study included 410 samples of shellfish (164 clams, 86 mussels, and 160 shrimps) collected from the three Governorates of the Suez Canal area. Harvesting water samples were collected from the three sites. All samples were collected during the warmest season (June, July, and August) through two years. Samples were processed, and the enriched samples were identified by plating onto TCBS agar. Presumptive V. parahaemolyticus colonies were selected, purified, and further identified by API20 and PCR techniques targeting toxR gene. The pathogenicity of the isolates was examined by detection of Thermostable Direct Haemolysin (TDH) and related Haemolysin (TRH) genes. Results revealed that the overall prevalence of V. parahaemolyticus in shellfish was 38/410 (9.27%), whereas in water was 12/48 (25%). Higher contamination rate was detected in shrimp (15%), and the highest prevalence was recorded in Ismailia governorate (12.2%). The detection rate of TDH and TRH genes among V. parahaemolyticus isolates was 21.05% and 5.26% consequently indicating its health hazards to the consumers. This study concluded that the examined shellfish may have the potential human health risk associated with the presence of pathogenic V. parahaemolyticus.
- Subjects
SUEZ Canal (Egypt); VIBRIO parahaemolyticus; SHELLFISH contamination; ZOONOSES; POLYMERASE chain reaction
- Publication
International Food Research Journal, 2018, Vol 25, Issue 6, p2375
- ISSN
1985-4668
- Publication type
Article