We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Culture-Independent Sequence-Based Metagenomics Approach to the Investigation of an Outbreak of Shiga-Toxigenic Escherichia coli O104:H4.
- Authors
Loman, Nicholas J.; Constantinidou, Chrystala; Christner, Martin; Rohde, Holger; Chan, Jacqueline Z.-M.; Quick, Joshua; Weir, Jacqueline C.; Quince, Christopher; Smith, Geoffrey P.; Betley, Jason R.; Aepfelbacher, Martin; Pallen, Mark J.
- Abstract
The article discusses research which examined the potential of metagenomics as an open-ended clinical discovery platform capable of identifying bacterial strains from an outbreak without laboratory culture. The study considers 45 samples from fecal specimens obtained from patients with diarrhea during the 2011 outbreak of Shiga-toxigenic Escherichia coli (STEC) in Germany. A draft genome of the STEC outbreak strain was obtained in phase 1, while the outbreak strain genome was recovered during phase 2. Findings showed sequences from the Shiga-toxin genes in 27 samples. Sequences from Clostridium difficile, Campylobacter jejuni and Salmonella enterica were obtained in phase 3.
- Subjects
METAGENOMICS; DIAGNOSIS of bacterial diseases; ESCHERICHIA coli diseases; BACTERIAL genomes; BACTERIAL toxins; CLOSTRIDIOIDES difficile; CAMPYLOBACTER jejuni; SALMONELLA enterica
- Publication
JAMA: Journal of the American Medical Association, 2013, Vol 309, Issue 14, p1502
- ISSN
0098-7484
- Publication type
Article
- DOI
10.1001/jama.2013.3231