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- Title
Automated Low Cost Method of Quantification of Hepatitis C Virus.
- Authors
Marikar, F. M. M. T.; Senevirathna, D.; Fernandopulle, N.
- Abstract
This paper describes the development of a Dig-dUTP based multiplex real time RT-PCR for the simultaneous detection of HCV viral amount in plasma samples. Viral genomes were identified in the same sample by Dig-dUTP PCR 216 bp region. Analysis of known scalar concentrations of reference plasma indicated that the multiplex procedure detects at least 500 copies/ml of HCV. In addition, we also assayed HCV viral load in eighty co-infected patients and in fifteen blood donors, confirming the sensitivity and specificity of the assay. This method may represent a useful alternative method for the detection of HCV co-infection, reliable for a rapid and relatively inexpensive screening of blood donors. The assay may be used to determine posttherapy viral clearance.
- Subjects
VIRAL genomes; HEPATITIS C virus; HEPATITIS viruses; BLOOD donors; MICROBIAL sensitivity tests; MEDICAL screening; PATIENTS
- Publication
Bangladesh Journal of Medical Science, 2015, Vol 14, Issue 3, p247
- ISSN
2223-4721
- Publication type
Article
- DOI
10.3329/bjms.v14i3.22270