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- Title
Biomarkers of oxidative stress and antioxidant defences as indicators of different disinfectants exposure in the heart of rainbow trout ( Oncorhynchus Mykiss Walbaum).
- Authors
Tkachenko, Halyna; Kurhaluk, Natalia; Grudniewska, Joanna
- Abstract
The aim of this study was to evaluate the impacts of different disinfectants' treatment using in aquaculture on the oxidative stress biomarkers such as thiobarbituric acid reactive substrates ( TBARS) and carbonyl derivatives of protein oxidative modification, as well as antioxidant defences [superoxide dismutase ( SOD), catalase ( CAT), glutathione reductase ( GR) and glutathione peroxidase ( GPx) activity] and total antioxidant capacity ( TAC) in the heart tissue of rainbow trout ( Oncorhynchus mykiss). In the disinfectants exposure, fish were treated to chloramine- T (final concentration 10 g m−3), chloride dioxide (5 g m−3), formalin (200 mL m−3) and CIP disinfectant based on peracetic acid and hydrogen peroxide (16 mL m−3) for 20 min and repeated three times every 3 days. Both chlorine dioxide and formalin treatment was indicated by a significant increase in the level of heart TBARS levels and carbonyl derivatives content and decreased SOD activity. Tissue oxidative stress biomarkers were unchanged upon chloramine- T or CIP disinfectant exposure. Increased oxidative stress could modify antioxidant defences, principally causing increased CAT activity in the heart tissue of formalin- or ClO2−-exposed fish. The correlation between oxidative stress biomarkers and GPx activity indicates that enzymes related to glutathione metabolism were responsible to formalin or ClO2−-induced oxidative stress. Hence, TBARS, carbonyl derivatives and antioxidant defences could be used as biomarkers in evaluating the toxicity of formalin and chlorine dioxide using as disinfectants to trout.
- Subjects
RAINBOW trout; OXIDATIVE stress; ANTIOXIDANTS; DISINFECTION &; disinfectants; ONCORHYNCHUS
- Publication
Aquaculture Research, 2015, Vol 46, Issue 3, p679
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/are.12214