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- Title
Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258.
- Authors
Wyres, Kelly L.; Gorrie, Claire; Edwards, David J.; Wertheim, Heiman F. L.; Li Yang Hsu; Nguyen Van Kinh; Zadoks, Ruth; Baker, Stephen; Holt, Kathryn E.
- Abstract
Klebsiellapneumoniae clonalgroup(CG)258, comprisingsequence types (STs)258,11,andclosely related variants, is associated with disseminationof the K.pneumoniae carbapenemase (KPC).Hospitaloutbreaks ofKPCCG258infections havebeenobserved globally and are very difficult to treat. As a consequence, there is renewed interest in alternative infection controlmeasures such as vaccines and phage or depolymerase treatments targeting the K. pneumoniae polysaccharide capsule. To date, 78 immunologically distinct capsule variants have been described in K. pneumoniae. Previous investigations of ST258 and a small number of closely related strains suggestedthat capsular variationwas limited within this clone;only twodistinctST258capsule polysaccharide synthesis (cps) locihave been identified, both acquired through large-scale recombination events (>50 kb). In contrast to previous studies, we report a comparative genomic analysis of the broader K. pneumoniae CG258 (n= 39). We identified 11 different cps loci within CG258, indicating that capsular switching is actuallycommon within the complex. Weobserved several insertion sequences (IS) within the cps loci, and show further intraclone diversification of two cps loci through IS activity. Our data also indicate that several large-scale recombinationeventshave shapedthegenomesofCG258, andthatdefinitionof thecomplex shouldbebroadenedto include ST395 (also reported to harborKPC).As only the second report of extensive intraclonal cps variation amongGram-negative bacterial species, our findings alter our understanding of the evolution of these organismsand have key implications for the design of controlmeasures targeting K. pneumoniae capsules.
- Subjects
POLYSACCHARIDES; PANDEMICS; KLEBSIELLA; ENTEROBACTERIACEAE; KLEBSIELLA pneumoniae
- Publication
Genome Biology & Evolution, 2015, Vol 7, Issue 5, p1267
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evv062