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- Title
Jasmonate-Responsive ERF Transcription Factors Regulate Steroidal Glycoalkaloid Biosynthesis in Tomato.
- Authors
Chonprakun Thagun; Shunsuke Imanishi; Toru Kudo; Ryo Nakabayashi; Kiyoshi Ohyama; Tetsuya Mor; Koichi Kawamoto; Yukino Nakamura; Minami Katayama; Satoko Nonaka; Chiaki Matsukura; Kentaro Yano; Hiroshi Ezura; Kazuki Saito; Takashi Hashimoto; Tsubasa Shoji
- Abstract
Steroidal glycoalkaloids (SGAs) are cholesterol-derived specialized metabolites produced in species of the Solanaceae. Here, we report that a group of jasmonate-responsive transcription factors of the ETHYLENE RESPONSE FACTOR (ERF) family (JREs) are close homologs of alkaloid regulators in Cathranthus roseus and tobacco, and regulate production of SGAs in tomato. In transgenic tomato, overexpression and dominant suppression of JRE genes caused drastic changes in SGA accumulation and in the expression of genes for metabolic enzymes involved in the multistep pathway leading to SGA biosynthesis, including the upstream mevalonate pathway. Transactivation and DNA-protein binding assays demonstrate that JRE4 activates the transcription of SGA biosynthetic genes by binding to GCC box-like elements in their promoters. These JRE-binding elements occur at significantly higher frequencies in proximal promoter regions of the genes regulated by JRE genes, supporting the conclusion that JREs mediate transcriptional co-ordination of a series of metabolic genes involved in SGA biosynthesis.
- Subjects
TOMATO yields; SOLANACEAE; JASMONATE; TRANSCRIPTION factors; PHYSIOLOGICAL effects of steroids; GLYCOALKALOIDS; BIOSYNTHESIS
- Publication
Plant & Cell Physiology, 2016, Vol 57, Issue 5, p961
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pcw067