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- Title
Dietary fish oil up-regulates cholesterol 7α-hydroxylase mRNA in mouse liver leading to an increase in bile acid and cholesterol excretion
- Authors
Bérard, Annie M.; Dumon, Marie-France; Darmon, Michel
- Abstract
To investigate the molecular events controlling reverse cholesterol transport, we compared gene expression of normal mouse liver to that of mice fed a long chain (LC) ω-3 fatty acid-enriched diet. Using cDNA microarrays, we assessed expression levels of 1176 genes, and we found that D-site binding protein (DBP) was three-fold increased in mice on a LC ω-3 fatty acid-rich diet compared to controls. DBP is known to increase transcriptional level of cholesterol 7α-hydroxylase (C7α), the rate-limiting enzyme for bile acid production and cholesterol excretion, and we found that C7α mRNA was also up-regulated by LC ω-3 fatty acids. Moreover, liver X receptor-α, another transcription factor up-regulating C7α, was three- to four-fold increased in liver of treated mice. On the other hand, we demonstrated that bile acid and cholesterol excretion were two-fold increased. These results show that LC ω-3 fatty acids control cholesterol metabolism in mice at a new endpoint.
- Subjects
CHOLESTEROL; HYDROLASES; FISH oils; GENE expression
- Publication
FEBS Letters, 2004, Vol 559, Issue 1-3, p125
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(04)00049-3