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- Title
Gene silencing by adenovirus-delivered siRNA
- Authors
Shen, Changxian; Buck, Andreas K.; Liu, Xiangwei; Winkler, Michael; Reske, Sven N.
- Abstract
RNA interference is the process that double-stranded RNA induces the homology-dependent degradation of cognate mRNA mediated by 21–23 nucleotide short interfering RNA (siRNA). Here, we describe a simple virus vector for efficient delivery of siRNA into mammalian cells utilizing the well-defined H1-RNA promoter and conventional adenovirus. In this pilot study, p53 was targeted by this vector. Our results demonstrate efficient and specific knock-down of p53 in breast cancer MCF-7 and lung carcinoma A549 cells and indicate a prospective application of this siRNA expressing recombinant adenovirus system in functional genomics, cancer gene therapy and virus inhibition.
- Subjects
GENE silencing; ADENOVIRUSES
- Publication
FEBS Letters, 2003, Vol 539, Issue 1-3, p111
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)00209-6