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- Title
The DMPC lipid phase transition influences differently the first and the second electron transfer reactions in bacterial reaction centers
- Authors
Taly, Antoine; Baciou, Laura; Sebban, Pierre
- Abstract
Photosynthetic reaction centers (RCs) from Rhodobacter sphaeroides were incorporated in dimyristoylphosphatidylcholine (DMPC) liposomes. The first and second electron transfer rates (kAB(1) and kAB(2), respectively) between the first and the second quinone electron acceptors have been measured as a function of temperature, across the phase transition of DMPC (23°C). The Eyring plots of kAB(1) display straight lines. In contrast, the Eyring plots for kAB(2) in proteoliposomes show a break at about 23.5°C. This physical discrimination between the two electron transfer reactions demonstrates that the stiffness of the lipid environment of the RCs and/or the protein–protein interactions influence the parameters governing kAB(2), but not the gating process limiting kAB(1).
- Subjects
PHOTOSYNTHETIC reaction centers; LIPOSOMES
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p91
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03635-9