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- Title
Allelic Losses in Oligodendroglial and Oligodendroglioma-like Neoplasms.
- Authors
Johnson, Mahlon D.; Vnencak-Jones, Cindy L.; Toms, Steven A.; Moots, Paul M.; Weil, Robert
- Abstract
Context.--Hemochromatosis tumors are heterogenous neoplasms with histologic features shared with other central nervous system tumors, such as dysembryoplastic neuroepithelial tumors. Objective.--We examined a series of tumors, identified as possessing oligodendroglial components at the time of intraoperative examination, to see if molecular subsets based on the oligodendroglial component could be recognized. Design.--DNA was extracted from fresh brain tumor tissue and corresponding peripheral blood or normal tissues. Genotypes for multiple loci were determined by polymerase chain reaction amplification using fluorescent-labeled primers for markers on chromosomes 1p, 17p, and 19q. Results.--Of the 12 oligodendrogliomas, 6 (60%) of 10 informative cases for 1p exhibited loss of heterozygosity (LOH). Six (50%) of 12 informative cases for 19q exhibited LOH. Each case also showed LOH at 1p. Three (25%) of 12 informative cases exhibited LOH at 17p for the dinucleotide repeat within the TP53 gene. In oligoastrocytomas, none of 4 informative cases showed LOH at 1p, 1 (25%) showed LOH at 19q, and 2 (50%) at 17p. One case also displayed microsatellite instability at 3 of 8 markers. In the 3 anaplastic oligodendrogliomas, 1 was not informative for 1p and none of the informative tumors exhibited LOH at 1p or 17p; 1 case (33%) exhibited LOH at 19q. Of the 14 informative anaplastic oligoastrocytomas, LOH was seen in 5 (36%) at both 1p and 19q and in 2 (14%) at 17p. Those with allelic loss at TP53 were astrocytoma predominant. No dysembryoplastic neuroepithelial tumors exhibited LOH at any marker on 1p, 17p, or 19q. Conclusions.--These findings suggest that routine screening for allelic losses, in samples intraoperatively determined to have an oligodendroglial component, will reveal prognostically or therapeutically relevant information in the majority of cases.
- Subjects
TUMORS; OLIGODENDROGLIA; NERVOUS system tumors; MICROSATELLITE repeats; NUCLEOTIDE sequence; POLYMERASE chain reaction
- Publication
Archives of Pathology & Laboratory Medicine, 2003, Vol 127, Issue 12, p1573
- ISSN
0003-9985
- Publication type
Article
- DOI
10.5858/2003-127-1573-alioao