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- Title
Bacteriophage T4 UvsW protein is a helicase involved in recombination, repair and the regulation of DNA replication origins.
- Authors
Carles-Kinch, Kelly; George, James W.; Kreuzer, Kenneth N.
- Abstract
Bacteriophage T4 UvsW protein is involved in phage recombination, repair and the regulation of replication origins. Here, we provide evidence that UvsW functions as a helicase. First, expression of UvsW allows growth of an (otherwise inviable) Escherichia coli recG rnhA double mutant, consistent with UvsW being a functional analog of the RecG helicase. Second, UvsW contains helicase sequence motifs, and a substitution (K141R) in the Walker ‘A’ motif prevents growth of the E.coli recG rnhA double mutant. Third, UvsW, but not UvsW K141R, inhibits replication from a T4 origin at which persistent RNA-DNA hybrids form and presumably trigger replication initiation. Fourth, mutations that inactivate UvsW and endonuclease VII (which cleaves DNA branches) synergistically block repair of double strand breaks. These in vivo results are consistent with a model in which UvsW is a DNA helicase that catalyzes branch migration and dissociation of RNA-DNA hybrids. In support of this model, a partially purified GST/UvsW fusion protein, but not a GST/UvsW K141R fusion, displays ssDNA-dependent ATPase activity and is able to unwind a branched DNA substrate.
- Subjects
BACTERIOPHAGES; PROTEINS; DNA replication; DNA helicases; GENETIC recombination; ESCHERICHIA coli; ISOMERASES
- Publication
EMBO Journal, 1997, Vol 16, Issue 13, p4142
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/16.13.4142