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- Title
Upconverting Nanoparticles as a New Bio-Imaging Strategy—Investigating Intracellular Trafficking of Endogenous Processes in Neural Tissue.
- Authors
Zajdel, Karolina; Janowska, Justyna; Frontczak-Baniewicz, Małgorzata; Sypecka, Joanna; Sikora, Bożena
- Abstract
In recent years, rare-earth-doped upconverting nanoparticles (UCNPs) have been widely used in different life sciences due to their unique properties. Nanoparticles have become a multifunctional and promising new approach to neurobiological disorders and have shown extraordinary application potential to overcome the problems related to conventional treatment strategies. This study evaluated the internalization mechanisms, bio-distribution, and neurotoxicity of NaYF4:20%Yb3+,2%Er3+ UCNPs in rat organotypic hippocampal slices. TEM results showed that UCNPs were easily internalized by hippocampal cells and co-localized with selected organelles inside neurons and astrocytes. Moreover, the UCNPs were taken into the neurons via clathrin- and caveolae-mediated endocytosis. Propidium iodide staining and TEM analysis did not confirm the adverse effects of UCNPs on hippocampal slice viability and morphology. Therefore, UCNPs may be a potent tool for bio-imaging and testing new therapeutic strategies for brain diseases in the future.
- Subjects
NERVE tissue; BRAIN diseases; NANOPARTICLES; PROPIDIUM iodide; LIFE sciences; NANOMEDICINE; SUPRACHIASMATIC nucleus; ASTROCYTES
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 2, p1122
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24021122