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- Title
Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol.
- Authors
von Schoenfeld, Anna; Bronsert, Peter; Poc, Michael; Fuller, Andrew; Filby, Andrew; Kraft, Stefan; Kurowski, Konrad; Sörensen, Kristin; Huber, Julia; Pfeiffer, Jens; Proietti, Michele; Stehl, Verena; Werner, Martin; Seidl, Maximilian
- Abstract
We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retrieval. Histomorphologic details are preserved, and crossreactivity and loss of signal intensity are not detectable. Combined with a DAB-based hydrophobic masking of metal-labeled primary antibodies, FOLGAS allows the extended use of CyTOF imaging in FFPE sections.
- Subjects
IMMUNOSTAINING; IMMUNOFLUORESCENCE; FORMALDEHYDE; IMMUNOHISTOCHEMISTRY; IMMUNOGLOBULINS
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 1, p223
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23010223