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- Title
Skeletal Muscle Na,K-ATPase as a Target for Circulating Ouabain.
- Authors
Kravtsova, Violetta V.; Bouzinova, Elena V.; Matchkov, Vladimir V.; Krivoi, Igor I.
- Abstract
While the role of circulating ouabain-like compounds in the cardiovascular and central nervous systems, kidney and other tissues in health and disease is well documented, little is known about its effects in skeletal muscle. In this study, rats were intraperitoneally injected with ouabain (0.1–10 µg/kg for 4 days) alone or with subsequent injections of lipopolysaccharide (1 mg/kg). Some rats were also subjected to disuse for 6 h by hindlimb suspension. In the diaphragm muscle, chronic ouabain (1 µg/kg) hyperpolarized resting potential of extrajunctional membrane due to specific increase in electrogenic transport activity of the α2 Na,K-ATPase isozyme and without changes in α1 and α2 Na,K-ATPase protein content. Ouabain (10–20 nM), acutely applied to isolated intact diaphragm muscle from not injected rats, hyperpolarized the membrane to a similar extent. Chronic ouabain administration prevented lipopolysaccharide-induced (diaphragm muscle) or disuse-induced (soleus muscle) depolarization of the extrajunctional membrane. No stimulation of the α1 Na,K-ATPase activity in human red blood cells, purified lamb kidney and Torpedo membrane preparations by low ouabain concentrations was observed. Our results suggest that skeletal muscle electrogenesis is subjected to regulation by circulating ouabain via the α2 Na,K-ATPase isozyme that could be important for adaptation of this tissue to functional impairment.
- Subjects
SKELETAL muscle; OUABAIN; MEMBRANE potential; SOLEUS muscle; ERYTHROCYTES; CENTRAL nervous system
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 8, p2875
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21082875