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- Title
A novel single amino acid deletion impairs fibronectin function and causes familial glomerulopathy with fibronectin deposits: case report of a family.
- Authors
dos Reis Monteiro, Maria Luíza Gonçalves; Custódio, Fabiano Bichuette; de Menezes Neves, Precil Diego Miranda; Ferreira, Frederico Moraes; Watanabe, Elieser Hitoshi; Lerário, Antônio Marcondes; de Araújo, Liliane Silvano; Balbo, Bruno Eduardo Pedroso; Pinto, Vívian Christine Dourado; Barbosa, Lívia Maria Gruli; de Paiva Marques, Vilmar; Machado, Juliana Reis; Reis, Marlene Antônia; Onuchic, Luiz Fernando
- Abstract
<bold>Background: </bold>Glomerulopathy with fibronectin deposits is an autosomal dominant disease associated with proteinuria, hematuria, hypertension and renal function decline. Forty percent of the cases are caused by mutations in FN1, the gene that encodes fibronectin.<bold>Case Presentation: </bold>This report describes two cases of Glomerulopathy with fibronectin deposits, involving a 47-year-old father and a 14-year-old son. The renal biopsies showed glomeruli with endocapillary hypercellularity and large amounts of mesangial and subendothelial eosinophilic deposits. Immunohistochemistry for fibronectin was markedly positive. Whole exome sequencing identified a novel FN1 mutation that leads to an amino-acid deletion in both patients (Ile1988del), a variant that required primary amino-acid sequence analysis for assessment of pathogenicity. Our primary sequence analyses revealed that Ile1988 is very highly conserved among relative sequences and is positioned in a C-terminal FN3 domain containing heparin- and fibulin-1-binding sites. This mutation was predicted as deleterious and molecular mechanics simulations support that it can change the tertiary structure and affect the complex folding and its molecular functionality.<bold>Conclusion: </bold>The current report not only documents the occurrence of two GFND cases in an affected family and deeply characterizes its anatomopathological features but also identifies a novel pathogenic mutation in FN1, analyzes its structural and functional implications, and supports its pathogenicity.
- Subjects
AMINO acids; HEMATURIA; TERTIARY structure; FIBRONECTINS; SEQUENCE analysis; FATHER-son relationship
- Publication
BMC Nephrology, 2019, Vol 20, Issue 1, pN.PAG
- ISSN
1471-2369
- Publication type
journal article
- DOI
10.1186/s12882-019-1507-7