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- Title
Studies on tracheorelaxant and anti-inflammatory activities of rhizomes of Polygonatum verticillatum.
- Authors
Khan, Haroon; Saeed, Muhammad; Mehmood, Malik Hassan; Rehman, Najeeb-ur; Muhammad, Naveed; Haq, Ikram-ul; Ashraf, Nadeem; El-Tahir, Kamal Eldin H.; Gilani, Anwarul-Hassan
- Abstract
Background: The present study describes the tracheorelaxant and anti-inflammatory effects of Polygonatum verticillatum which may support its medicinal use in hyperactive airway complaints and inflammatory disorders. Methods: The tracheorelaxant activity of crude extract of the rhizomes of P. verticillatum (PR) was assessed in isolated guinea-pig tracheal tissues immersed in tissue organ bath filled with Tyrode’s solution and a continuous supply of carbogen gas (95% O2 and 5% CO2). The contractile and relaxant responses of the tissue were measured using isometric transducers coupled with Power-Lab data acquisition system. The anti-inflammatory effect was evaluated in carrageenan-induced rat paw edema model, while the lipoxygenase inhibitory activity was performed in the in-vitro assay. Various chromatographic and spectroscopic techniques were used for the isolation and characterization of pure molecules. Results: In isolated guinea-pig tracheal preparations, PR caused complete inhibition of the high K+ (80 mM) and carbachol-induced contractions however, it was more potent against K+ than CCh, similar to verapamil. Pretreatment of the tissue with PR, displaced the Ca2+ concentration-response curves to the right, similar to that induced by verapamil, indicating the presence of Ca2+ channel blocking like activity. When tested on carrageenaninduced rat paw edema, PR demonstrated a marked reduction in edema with 65.22% protection at 200 mg/kg, similar to aspirin. In the in-vitro assay, PR showed lipoxygenase inhibitory activity (IC50: 102 ± 0.19 μg/mL), similar to baicalein. Bioactivity-guided fractionation led to the isolation of 2-hydroxybenzoic acid and β-sitosterol. Conclusions: These results indicate that the plant possesses tracheorelaxant, mediated possibly through a Ca2+ channel blockade mechanism, and anti-inflammatory activities, which may explain the medicinal use of this plant in airway disorders and inflammation.
- Subjects
THERAPEUTIC use of plant extracts; ANIMAL experimentation; BIOLOGICAL assay; BIOLOGICAL models; CHROMATOGRAPHIC analysis; EDEMA; GUINEA pigs; INFLAMMATION; MEDICINAL plants; RATS; PLANT roots; STATISTICS; TRACHEA; PLANT extracts; DATA analysis; IN vitro studies
- Publication
BMC Complementary & Alternative Medicine, 2013, Vol 13, Issue 1, p197
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/1472-6882-13-197