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- Title
Antimicrobial activities of endophytic fungi isolated from Ophiopogon japonicus (Liliaceae).
- Authors
Liang, Hanqiao; Xing, Yongmei; Chen, Juan; Zhang, Dawei; Guo, Shunxing; Wang, Chunlan
- Abstract
Background: Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Endophytes provide an abundant reservoir of bioactive metabolites for medicinalexploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional Chinese medicinal plant used for eliminating phlegm, relieving coughs, latent heat in the lungs, and alleviating diabetes mellitus. We investigated the antimicrobial activities of 30 strains of O. japonicus.Methods: Fungal endophytes were isolated from roots and stems of O. japonicus collected from Chongqing City,southwestern China. Mycelial extracts (MC) and fermentation broth (FB) were tested for antimicrobial activity using peptide deformylase (PDF) inhibition fluorescence assays and MTT cell proliferation assays.Results: A total of 30 endophytic strains were isolated from O. japonicus; 22 from roots and eight from stems.53.33% of the mycelial extracts (MC) and 33.33% of the fermentation broths (FB) displayed potent inhibition of PDF.80% of MC and 33.33% of FB significantly inhibited Staphylococcus aureus. 70% of MC and 36.67% of FB showed strong activities against Cryptococcus neoformans. None showed influence on Escherichia coli.Conclusion: The secondary metabolites of endophytic fungi from O. japonicus are potential antimicrobial agents.
- Subjects
ANTI-infective agents; COLORIMETRY; DRUG design; FLUORESCENCE spectroscopy; FUNGI; CHINESE medicine; RESEARCH funding; PLANT roots; PLANT stems; PLANT extracts; IN vitro studies
- Publication
BMC Complementary & Alternative Medicine, 2012, Vol 12, Issue 1, p238
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/1472-6882-12-238