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- Title
Puroindoline-a and α1-purothionin form ion channels in giant liposomes but exert different toxic actions on murine cells.
- Authors
Llanos, Paola; Henriquez, Mauricio; Minic, Jasmina; Elmorjani, Khalil; Marion, Didier; Riquelme, Gloria; Molgó, Jordi; Benoit, Evelyne
- Abstract
Puroindoline-a (PIN-a) and α1-purothionin (α1-PTH), isolated from wheat endosperm of Triticum aestivum sp., have been suggested to play a role in plant defence mechanisms against phytopathogenic organisms. We investigated their ability to form pores when incorporated into giant liposomes using the patch-clamp technique. PIN-a formed cationic channels (≈ 15 pS) with the following selectivity K+ > Na+ ≫ Cl–. Also, α1-PTH formed channels of ≈ 46 pS and 125 pS at +100 mV, the selectivity of which was Ca2+ > Na+ ≈ K+ ≫ Cl– and Cl– ≫ Na+, respectively. In isolated mouse neuromuscular preparations, α1-PTH induced muscle membrane depolarization, leading to blockade of synaptic transmission and directly elicited muscle twitches. Also, α1-PTH caused swelling of differentiated neuroblastoma NG108-15 cells, membrane bleb formation, and disorganization of F-actin. In contrast, similar concentrations of PIN-a had no detectable effects. The cytotoxic actions of α1-PTH on mammalian cells may be explained by its ability to induce cationic-selective channels.
- Subjects
WHEAT; PLANT defenses; PHYTOPATHOGENIC microorganisms; LIPOSOMES; NEURAL transmission; NEUROBLASTOMA
- Publication
FEBS Journal, 2006, Vol 273, Issue 8, p1710
- ISSN
1742-464X
- Publication type
Article
- DOI
10.1111/j.1742-4658.2006.05185.x