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- Title
Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins,Castanea crenataagglutinin andCycas revolutaleaf lectin.
- Authors
Nakamura, Sachiko; Yagi, Fumio; Totani, Kiichiro; Ito, Yukishige; Hirabayashi, Jun
- Abstract
Lectins belonging to the jacalin-related lectin family are distributed widely in the plant kingdom. Recently, two mannose-specific lectins having tandem repeat-type structures were discovered inCastanea crenata(angiosperm) andCycas revoluta(gymnosperm). The occurrence of such similar molecules in taxonomically less related plants suggests their importance in the plant body. To obtain clues to understand their physiological roles, we performed detailed analysis of their sugar-binding specificity. For this purpose, we compared the dissociation constants (Kd) ofCastanea crenataagglutinin (CCA) andCycas revolutaleaf lectin (CRLL) by using 102 pyridylaminated and 13p-nitrophenyl oligosaccharides with a recently developed automated system for frontal affinity chromatography. As a result, we found that the basic carbohydrate-binding properties of CCA and CRLL were similar, but differed in their preference for larger N-linked glycans (e.g. Man7–9 glycans). While the affinity of CCA decreased with an increase in the number of extendedα1–2 mannose residues, CRLL could recognize these Man7–9 glycans with much enhanced affinity. Notably, both lectins also preserved considerable affinity for mono-antennary, complex type N-linked glycans, though the specificity was much broader for CCA. The information obtained here should be helpful for understanding their functionsin vivoas well as for development of useful probes for animal cells. This is the first systematic approach to elucidate the fine specificities of plant lectins by means of high-throughput, automated frontal affinity chromatography.
- Subjects
LECTINS; PLANT lectins; MANNOSE; IMMUNOGLOBULINS; ANGIOSPERMS; GYMNOSPERMS; CHROMATOGRAPHIC analysis
- Publication
FEBS Journal, 2005, Vol 272, Issue 11, p2784
- ISSN
1742-464X
- Publication type
Article
- DOI
10.1111/j.1742-4658.2005.04698.x