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- Title
Idiosyncratic tuning of tRNAs to achieve uniform ribosome binding.
- Authors
Olejniczak, Mikoaj; Dale, Taraka; Fahlman, Richard P; Uhlenbeck, Olke C
- Abstract
The binding of seven tRNA anticodons to their complementary codons on Escherichia coli ribosomes was substantially impaired, as compared with the binding of their natural tRNAs, when they were transplanted into tRNA2Ala. An analysis of chimeras composed of tRNA2Ala and various amounts of either tRNA3Gly or tRNA2Arg indicates that the presence of the parental 32-38 nucleotide pair is sufficient to restore ribosome binding of the transplanted anticodons. Furthermore, mutagenesis of tRNA2Ala showed that its highly conserved A32-U38 pair serves to weaken ribosome affinity. We propose that this negative binding determinant is used to offset the very tight codon-anticodon interaction of tRNA2Ala. This suggests that each tRNA sequence has coevolved with its anticodon to tune ribosome affinity to a value that is the same for all tRNAs.
- Subjects
TRANSFER RNA; RIBOSOMES; ORGANELLES; NUCLEOPROTEINS; PROTOPLASM; RNA
- Publication
Nature Structural & Molecular Biology, 2005, Vol 12, Issue 9, p788
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb978