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- Title
Small ruminant lentivirus capsid protein (SRLV-p25) antigenic structural prediction and immunogenicity to recombinant SRLV-rp25-coupled to immunostimulatory complexes based on glycyrrhizinic acid.
- Authors
Azucena Castañeda-Montes, María; Sandra Cuevas-Romero, Julieta; Luis Cerriteño-Sánchez, José; Ávila-De la Vega, Lucero de María; Bryan García-Cambrón, José; Ramírez-Álvarez, Hugo
- Abstract
Small ruminant lentiviruses (SRLV) infect sheep and goats resulting in significant economic losses. This study evaluated for the first time the predicted conformational structure of the SRLV-capsid-protein 25 (SRLV-p25) and analyzed the antigenicity of recombinant protein (SRLV-rp25) in mice by coupling to an immunostimulatory complexes based on glycyrrhizinic acid liposomes (GAL) and tested plasma from goats and sheep naturally infected. Analysis in silico and conformational structure of SRLV-p25 (genotype B-FESC-752) showed similar characteristics to other lentiviral capsids. The efficient expression of SRLV-rp25 was confirmed by Western blot. The humoral immune responses in mice showed an increased level of antibodies from day 21 to 35 of the SRLV-r p25-GAL and SRLV-rp25-ISCOM® groups and the cellular immune response showed no significant difference in IL-10 levels (P > .05), however, a significant difference (P < .001) was observed when comparing SRLV-rp25-GAL with SRLV-rp25 groups. Immunoreactivity toward SRLV- r p25 revealed 61% of positive samples from naturally infected goats and sheep.
- Subjects
IMMUNE response; RUMINANTS; LENTIVIRUSES; RECOMBINANT proteins; PROTEINS
- Publication
Bioscience, Biotechnology & Biochemistry, 2023, Vol 87, Issue 3, p267
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbac206