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- Title
Influence of plant growth regulators on shoot proliferation and secondary metabolite production in micropropagated Huernia hystrix.
- Authors
Amoo, Stephen; Staden, Johannes
- Abstract
Although the effectiveness of topolins in plant tissue culture systems has recently been highlighted, there is a dearth of information on their interactions with auxins in relation to shoot organogenesis and secondary metabolite production. The current study evaluated the role of topolins singly or in combination with an auxin in comparison to 6-benzyladenine (BA) on shoot proliferation and secondary metabolite production of Huernia hystrix, a medicinal and ornamental stem-succulent of the endemic flora of southern Africa. Meta-topolin ( mT) was more effective in improving shoot proliferation and phenolic production compared to BA. In general, the exogenous addition of α-naphthalene acetic acid (NAA) significantly increased shoot proliferation. The highest number of regenerated shoots (12.2 ± 0.98 shoots per explant) was recorded with medium containing 20 μM mT supplemented with 10 μM NAA and was three-times higher when compared to the treatments with cytokinin only. This suggests a synergistic interaction of auxin with cytokinin. On the other hand, supplementation with low NAA concentrations resulted in reduced in vitro flavonoid production in most cases, when compared to treatments with cytokinin only. Moreover, differences in cytokinin concentrations (even when used in combination with NAA equimolar concentrations) significantly affected secondary metabolite production in some cases. The current findings highlighted the differential effects of auxin-cytokinin interactions on shoot proliferation and the production of secondary metabolites in H. hystrix.
- Subjects
AFRICA; PLANT regulators; PLANT metabolites; HUERNIA; PLANT tissue culture; PLANT biotechnology
- Publication
Plant Cell, Tissue & Organ Culture, 2013, Vol 112, Issue 2, p249
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-012-0230-x