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- Title
Indirect shoot organogenesis from leaves of Dieffenbachia cv. Camouflage.
- Authors
Xiuli Shen; Michael Kane
- Abstract
Abstract A novel protocol for indirect shoot organogenesis of Dieffenbachia cv. Camouflage was established using leaf explants excised from in vitro shoot cultures. The frequency of callus formation reached 96% for explants cultured on Murashige and Skoog (1962) basal medium supplemented with 5 μM thidiazuron and 1 μM 2,4-dichlorophenozyacetic acid. The number of shoots regenerated was high, with up to 7.9 shoots produced per callus cultured on basal medium supplemented with 40 μM N 6-(Δ2-isopentenyl)adenine and 2 μM indole-3-acetic acid. Regenerated shoots rooted well in a soilless substrate, acclimatized ex vitro at 100%, and grew vigorously under shaded greenhouse conditions. Somaclonal variations in leaf variegation, color, and morphology have been observed in regenerated plants.
- Subjects
MORPHOGENESIS; DIEFFENBACHIA; CAMOUFLAGE (Biology); PLANT morphology
- Publication
Plant Cell, Tissue & Organ Culture, 2007, Vol 89, Issue 2/3, p83
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-007-9214-7