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- Title
The application of quantitative NMR for the facile, rapid and reliable determination of clindamycin phosphate in a conventional tablet formulation.
- Authors
Liang, Xianrui; Du, Liping; Su, Feng; Parekh, Harendra S.; Su, Weike
- Abstract
Spectroscopic tools such as NMR can be applied to the quantitative analysis of active pharmaceutical ingredients with relative ease and accuracy. Here, we demonstrate the quantification of clindamycin phosphate (CLP) in a conventional tablet formulation, performed using potassium hydrogen phthalate (KHP) as the internal standard and deuterium oxide (D2O) as the NMR solvent. The methyl protons signal of CLP at 0.72 ppm (triplet) relative to the signal of KHP at 7.37-7.40 ppm (multiplet) was used for quantification purposes using 1H NMR. This method was shown to be specific and linear ( r = 0.9997) within the CLP concentration range from 7.2 to 23.1 mg per 0.5 ml of D2O. The maximum relative standard deviation (RSD) of accuracy and precision was calculated at 0.39% and 0.64%, respectively. The limits of detection (LOD) and quantification were 0.04 and 0.11 mg/ml, respectively. The method was highly stable with a calculated RSD of 0.03%. The robustness of the method was demonstrated by changing four different parameters, and the difference among each parameter was ≤ 0.78%. The findings of this work were in good agreement with previously reported conventional HPLC-based approaches, highlighting its applicability in the determination of other active pharmaceutical ingredients in conventional formulations for quality control purposes. Copyright © 2014 John Wiley & Sons, Ltd.
- Subjects
CLINDAMYCIN; DEUTERIUM oxide; STANDARD deviations; HIGH performance liquid chromatography; ASPIRIN; PHENACETIN; CHEMICAL shift (Nuclear magnetic resonance)
- Publication
Magnetic Resonance in Chemistry, 2014, Vol 52, Issue 4, p178
- ISSN
0749-1581
- Publication type
Article
- DOI
10.1002/mrc.4048