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- Title
WHO grade associated downregulation of MHC class I antigen-processing machinery components in human astrocytomas: does it reflect a potential immune escape mechanism?
- Authors
Mehling, Matthias; Simon, Perikles; Mittelbronn, Michel; Meyermann, Richard; Ferrone, Soldano; Weller, Michael; Wiendl, Heinz
- Abstract
Defects of major histocompatibility complex (MHC) class I antigen-processing machinery (APM) components have been shown to contribute to immune escape of malignant cells. We investigated the expression of APM components in astrocytomas without detectable defects in HLA class I antigen expression and correlated it with grade of malignancy. Quantitative immunohistochemical analysis of astrocytomas revealed reduced expression of the cytosolic proteasome subunit low molecular weight protein 2 (LMP2), the endoplasmatic reticulum (ER) transporter associated with antigen processing-1 (TAP1), and the ER chaperone β2-microglobulin (β2m) in astrocytoma cells when compared to astrocytes from nonpathological brain. Among human WHO grade II–IV astrocytomas, downregulation of LMP2, TAP1 and β2m correlated with grade of malignancy. Furthermore, astrocytoma cell lines ( n = 12) expressed all APM components analyzed at levels comparable to dendritic cells (DC), which were used for comparative purposes. However, upregulation of β2m after stimulation with inflammatory cytokines was significantly lower in astrocytoma cell lines than in control cells. Our results support the hypothesis that coordinated downregulation or impaired upregulation of certain HLA class I APM components may serve as a mechanism for astrocytoma cells to evade the host’s immune response, even if HLA class I antigen surface expression is not altered.
- Subjects
IMMUNE response; CANCER cells; DENDRITIC cells; CYTOKINES; MAJOR histocompatibility complex; IMMUNOGENETICS
- Publication
Acta Neuropathologica, 2007, Vol 114, Issue 2, p111
- ISSN
0001-6322
- Publication type
Article
- DOI
10.1007/s00401-007-0231-8