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- Title
Control of Somatic Cell Mitosis by Simulated Changes in the Transmembrane Potential Level.
- Authors
Cone, Jr., C.D.; Tongier, Jr., M.
- Abstract
Experiments designed to test the premis that variations in intracellular ionic concentrations accompanying different levels of the electrical transmembrane potential difference might serve as a mechanism for control of mitosis in somatic cells have yielded results supporting the validity of the premise Employing naturally synchronized CHO cells in vitro and test media designed to produce intracellular concentrations of Na+, K+, and CI- approximating those which would exist at various naturally-generated Em levels in vivo, it was found that mitosis was completely, but reversibly, blocked in both short and long-term tests by simulated Em levels of --70 mV and greater. This blockage was furthermore found to result from complete, but reversible, prevention of DNA synthesis. These findings that simulated high Em levels introduce a reversible G1 mitotic block in somatic cells in vitro lend credence to the supposition that natural modulation of cellular Em levels in vivo may constitute a basic mechanism for mitosis control in vivo. Copyright © 1971 S. Karger AG, Basel
- Publication
Oncology, 1971, Vol 25, Issue 2, p168
- ISSN
0030-2414
- Publication type
Article
- DOI
10.1159/000224567