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- Title
Toxoplasma gondii 529 baz çifti büyüklüğünde tekrar bölgesine (RE) özgü hızlı döngü aracılı izotermal amplifikasyon testinin geliştirilmesi ve analitik hassasiyetinin belirlenmesi
- Authors
Karakavuk, Muhammet; Can, Hüseyin; Karakavuk, Tuğba; Gül, Ceren; Alak, Sedef Erkunt; Gül, Aytül; Döşkaya, Aysu Değirmenci; Ün, Cemal; Gürüz, Adnan Yüksel; Döşkaya, Mert
- Abstract
Aim: Toxoplasma gondii is a protozoon that causes disease in humans and warm-blooded animals. The aim of this study is to determine the analytical sensitivity of the fluorescence-based rapid loop mediated isothermal amplification (LAMP) test developed by designing primers specific to T. gondii repeat region (RE) for the diagnosis of toxoplasmosis. Materials and Methods: T. gondii RE obtained with PCR was cloned into vector pCR2.1-TOPO. The analytical sensitivity of the LAMP assay developed using specific primers was determined by serial dilutions of the pCR2.1-RE vector. Results: It was determined that the analytical sensitivity of the LAMP test developed with primers designed specific to T. gondii RE gene was 10 copy plasmid / reaction. Conclusion: Since the RE region repeats 200-300 times in the T. gondii gene, the test developed has been calculated to have a detection limit of 0.05 tachyzoites/reaction. The LAMP test is predicted to be promising because it is easy, cheap, fast and suitable for field conditions and is more sensitive than other molecular tests.
- Publication
Ege Journal of Medicine, 2021, Vol 60, Issue 3, p269
- ISSN
1016-9113
- Publication type
Article
- DOI
10.19161/etd.990702