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- Title
MICROSCOPIC AND MOLECULAR DIAGNOSTIC STUDY OF BABESIA SPECIES IN CATTLE IN WASIT PROVINCE, IRAQ.
- Authors
Al-Abedi, Ghassan J. K.; Al-Al-Amery, Amer Murhum
- Abstract
Babesia species are intraerythrocytic protozoan parasites, which represent a serious threat to the livestock industry and cause great economic losses. To confirm Babesia species circulate in Iraqi cattle, the present study aimed for microscopic and molecular investigation of Babesia spp. and molecular genetics confirm of B. bovis, B. divergens and B. bigemina. For that, a total 180 cattle of variable ages and of both sexes were selected randomly from different areas in Wasit province. 180 blood smears, 12.78% cattle were positives microscopically to Babesia; involving single and multiple infections inside and outside of host RBCs. Of 100 DNA samples, 51% cattle were positives molecularly to Babesia. Also, three types of Babesia were detected including B. bovis (70.97%), B. bigmina (25.81%) and B. divergens (3.23%). Concerning to mode of infection, single infection appeared more significant (78.43%) than mixed. In addition, B. bovis (64.71%), B. bigmina (9.8%), and B. divergens (3.92%) were detected in single infections; whereas, only B. bovis and B. bigemina (21.57) reported in mixed infections. For confirmation, two from each positive local B. bovis, B. bigmina and B. divergens strains were reported in GenBank-NCBI under an accession numbers of MN124162.1, MN124163.1; MN124164.1, MN124165.1 and MN124166.1, MN124167.1, respectively. NCBI-BLAST homology sequence identity showed that the local B. bovis strains (IQK-Cattle No.1 and IQK-Cattle No.2) were respectively identical at 99.74% and 99.73%to the global USA isolate, B. bovis clone USDACL8AI (MH046903.1). For B. bigemina, local strains (IQK-Cattle No.1 and IQK-Cattle No.2) were respectively identical at 99.39% and 99.38% to the global Turkey isolate, B. bigemina Konya 3 (KT220511.1) isolate. For B. divergens,local strains (IQKCattle No. 1 and IQK-Cattle No. 2) were respectively identical at 99.29% and 98.56% to the global USA isolate, B. divergens clone BAB105 (AY046576.1) isolate.
- Subjects
IRAQ; MOLECULAR genetics; FINANCIAL crises; BABESIA bigemina; POLYMERASE chain reaction
- Publication
Biochemical & Cellular Archives, 2020, Vol 20, p3569
- ISSN
0972-5075
- Publication type
Article