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- Title
日粮钙磷水平对马岗鹅生长性能及肝脏基因表达的影响.
- Authors
李虹仪; 周润盛; 梁笑玲; 张楚玥; 吕祺欣; 杨长华; 张茂
- Abstract
To study the effect of calcium and phosphorus levels in diet on growth performance and liver metabolism related genes in Magang geese, a total of forty eight Magang geese of 50-day-old were randomly divided into three groups, with male and female in half. Each group had two replicates with eight geese per replicate, every two geese were kept in one cage. On the basis of the same diet, 0%, 0.15% and 0.3% calcium hydrogen phosphate were added respectively, each group was pre-fed for five days and fed for twenty-eight days, during which the daily feed intake was recorded, fecal samples and blood samples were collected regularly, and the content of calcium and phosphorus in geese samples were determined. At the end of the experiment, eight Magang geese were randomly selected from each group for slaughter and the slaughter performance and phosphorylase activity were measured. The liver tissue of each geese were collected for transcriptome sequencing, fluorescence quantitative PCR and enzyme activity detection. The results showed that the feed intake of control group was significantly higher than that of the other two groups (P<0.05). The average daily gain and carcass traits were significantly higher than that of 0.15% level group, with no significant difference from 0.3% level group (P >0.05). In addition, there was no significant difference in the content of calcium and phosphorus in blood among the three groups (P>0.05), but the content of fecal calcium increased with the increase of calcium and phosphorus level (P<0.05). Transcriptome sequencing results showed that there was less difference between control group and 0.15% level group, but more significant difference between control group and 0.3% level group. And most of the differently expressed genes were enriched in sugar metabolism pathway, which was consistent with the quantitative PCR results. Also the activity of phosphorylases, the glucose metabolism-related enzyme, decreased with the increase of calcium and phosphorus levels (P<0.05). In a word, the level of calcium and phosphorus in the diet will affect the food intake of Magang geese, and it will also affect the sugar and lipid metabolism in the liver.
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 11, p2533
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.20221657