We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
玫烟色棒束孢转录组测序及潜在致病相关基因分析.
- Authors
谢梅琼; 王龙江; 何余容; 吕利华
- Abstract
In order to explore the expression patterns of transcriptome genes of Isaria fumosorosea and to provide data for the functional study of I. fumosorosea, the transcriptom of I. fumosorosea IFCF01 was sequenced and analyzed using high-throughput non-parametric sequencing technology. A total of 540 896 444 clean reads were obtained by transcriptome sequencing. A total of 36 466 unigenes with the total length of 34 551 468 bp and an average length of 947 bp were obtained after assembly. The functional annotation showed that a total of 22 741 unigenes were annotated in 6 major databases,such as Nr, GO, COG and KEGG, accounting for 62.36% of the total unigenes. A total of 21 737 unigenes were annotated in the Nr database, with the highest number of anotation to Beauveria bassiana. 10 888 unigenes were annotated to 25 functional categories in COG database, and the number of genes involved in general function predicted only was the largest, matching on 4 060 unigenes. The GO annotation shoewed that a total of 9 617 unigenes were annotated, distributed in 49 subcategories of three functional modules: biological process function, molecular function and cell composition. A total of 14 364 unigenes were annotated in the KEGG database, involving 108 KEGG metabolic pathways. With the help of pathogen-host interaction gene database, a total of 86 unigenes associated with the potential pathogenicity of I. fumosorosea were identified. A total of 8 572 simple sequence repeats (SSR) loci were detected in transcriptome data of I. fumosorosea, with a frequency of 18.18%. The results of this study could lay the foundation for gene resources mining, functional gene research and SSR molecular markers for I. fumosorosea.
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 9, p2169
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.20221707