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- Title
基于LAMP的果蔬斯高维尔炭疽菌可视化检测方法.
- Authors
罗跃; 吴小毛; 刘阿丽; 姚小龙; 李荣玉; 耿广东
- Abstract
To provide an efficient, convenient, specific and visual detection method for the detection of Colletotrichum scovillei of fruit and vegetable, taking the ITS sequence of the internal transcription spacer of C. scovillei of fruit and vegetable as the target gene, 4 LAMP primers and 1 loop primer were designed. By choice the primer ratio, reaction time, reaction system and other conditions, a LAMP detection method with specific amplification for C. scovillei was established. The results of the specificity test show that the established LAMP reaction system was amplified for 60 min at the optimal reaction temperature of 65 ℃. The reaction system using C. scovillei DNA as a template was used as the indicator calcein and hydroxynaphthol. Under the action of blue, green and blue appear, respectively. The reaction result was positive, and the amplified product was detected as a ladder band by 2% agarose gel electrophoresis. The reaction system and blank control using the DNA of other 8 control strains as templates, they were orange and purple respectively, and both were negative reactions, and no amplified bands appeared, indicating that the LAMP detection method is highly specific to C. scovillei of fruit and vegetable.
- Publication
Acta Agriculturae Zhejiangensis, 2022, Vol 34, Issue 1, p98
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2022.01.12