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- Title
Comparison of Green Technology Supercritical Fluid Extraction with Conventional Extraction Technique for Betulinic Acid, A Potential Anticancer Agent, from Dillenia indica Linn. Bark using Experimental Design.
- Authors
Gandhi, Dipal; Mehta, Priti
- Abstract
Introduction: Betulinic acid has promising anticancer activity and present in appreciable quantity in Dillenia indica Linn bark commonly known as 'Karmal'. For the separation of betulinic acid from the bark of Dillenia indica Linn, supercritical fluid extraction was examined and compared to conventional extraction methods. The present investigation's goal was to isolate betulinic acid in pure form and compare ancient and novel extraction procedure. Methods: Soxhlet extraction followed by separation with column chromatography was applied and yield obtained was compared with super critical fluid extraction. The optimum extraction conditions for betulinic acid were also investigated by supercritical fluid extraction (SFE) using response surface methodology based on 3 factor 3 level Box-Behnken experimental design. Experiment design for SFE was performed to evaluate the combination effect of three independent variables like co-solvent concentration, temperature (35-60°C) and pressure (100-200 bar). Betulinic acid obtained was quantified using developed High Performance Thin Layer Chromatography method. Results: Analysis of variance showed that the "p-value" of SFE 0.0141 which indicate that models were statistically significant (p<0.05). and "coefficient of determination" (R2 value) is 0.94 which indicate that the model showed the goodness of fit. The optimum conditions for the efficient super critical fluid extraction of betulinic acid were co-solvent concentration 10%, extraction temperature 50°C and extraction pressure 200bar. Conclusion: Soxhlet extraction technique followed by column chromatography is advantageous because SFE require specialized equipment while the conventional method required more amount of chemicals and reagents. Application of chemo metric tools in optimization of methods is that it reduces the number of experiments, reagent consumption and tedious laboratory work. According to the results of the SFE experimental design, the co-solvent concentration and extraction pressure have the largest influence and improve the percentage yield, while the extraction temperature has a negative impact and decreases the total yield.
- Subjects
BETULINIC acid; SUPERCRITICAL fluid extraction; EXTRACTION techniques; GREEN technology; EXPERIMENTAL design
- Publication
Malaysian Journal of Medicine & Health Sciences, 2024, Vol 20, p18
- ISSN
1675-8544
- Publication type
Article