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- Title
Metabolic Alteration in Streptozotocin Induced Obese-Diabetic Rat Model Treated with Momordica charantia Extract Via 1H-NMR-Based Metabolomics.
- Authors
Khatib, Alfi; Ahmed, Qamar Uddin; Uzir, Bisha Fathamah; Hamid, Azizah; Ismail, Amin; Murugesu, Suganya; Taher, Muhammad; Saiman, Mohd Zuwairi; Ichwan, Muhammad; Primaharinastiti, Riesta; Selamat, Jinap; El-Seedi, Hesham R.; Peruma, Vikneswari
- Abstract
Introduction: Momordica charantia Linn (Cucurbitaceae) is used in folk medicine to treat various diseases including diabetes mellitus. It has been widely commercialised based on traditional usage as an antidiabetic product. The study investigates antidiabetic activity of M. charantia fruit through proton-nuclear magnetic resonance (1H-NMR) spectroscopy-based metabolomics approach. Methods: The 80 % ethanolic extract of M. charantia was force fed to obese-diabetic (ob-db) rats. Rats were divided into four groups: healthy, ob-db treated with 300 mg/kg bw metformin, ob-db treated with 300 mg/kg bw of M. charantia and ob-db rats. The dipeptidyl peptidase-IV (DPP-IV) inhibitory and 3T3-L1-cell glucose uptake activities were also tested for the same extract to explain its mode of action in relation to the metabolomics results. Results: The results showed that administration of the 80% ethanolic extract of 300 mg/kg bw for four weeks significantly (P < 0.05) reduced the blood glucose level. The identified biomarkers in serum and urine were 2-hydroxybutyrate, leucine, adipate, alanine, acetate, succinate, 2-oxoglutarate, dimethylamine, creatine, creatinine, betaine, glucose, taurine, phenylacetylglycine, allantoin and hippurate. Conclusion: The metabolomics approach indicated that the 80% ethanolic extract of M. charantia fruit and metformin have altered energy, amino acid, purine, creatine, bile, and gut microflora metabolisms of serum and urine profiles of the streptozotocin ob-db rats. Furthermore, it was found that the energy metabolism was ameliorated through the improvement of 3T3-L1-cell glucose uptake and inhibition DPP-IV.
- Subjects
MOMORDICA charantia; STREPTOZOTOCIN; METABOLOMICS; ANIMAL disease models; BLOOD sugar; NONNUTRITIVE sweeteners
- Publication
Malaysian Journal of Medicine & Health Sciences, 2023, Vol 19, p18
- ISSN
1675-8544
- Publication type
Abstract