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- Title
In vitro Inhibitory Activity of Shisandra chinensis and Polygonatum sibiricum against Vibrio harveyi and Its Biof lms.
- Authors
Zhifeng HUANG; Huanying PANG; Yang HUANG; Yucong HUANG; Yishan LU; Jichang JIAN
- Abstract
[ Obbective] This study aimed to analyze the in vitro inhibitory activity of Shisandra chinensis and Polygonatum sibiricum against Vibrio harveyi and its biofilms. [Result] By a a r diffusion test, in vitro inhibitory activity of S. chinensis a d P. sibiricum against V harveyi was investigated. The minima inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of S. chinensis and P. sibiricum against V. harveyi were determined by doubling dilution method. The inhibitory activity of S. chinensis a d P. sibiricum on the formation of V. harveyi biofilms was evaluated by modified MTT asay. [Result] Both S. chinensis and P. siiiricum had inhibitory activity aganst V harveyi. The inhibition zone diameter of S. chinensis aganst V harveyi was 17.95 mm; MIC and MBC of S. chinensis were both 3. 125 mg/ml. The inhibition zone diameter of P. sibiricum against V harveyi was 12. 22 mm; MIC and MBC of P. sibiricum were 3. 125 and 6.250 mg/ml, respectively. When the concentration was higher than 6.25 mg/ml, S. chinensis decoction had extremely significat inhibitory activity against V. harveyi (P <0. 01) ; when the concentration was higher t h a 3. 125 mg/ml, P. sirium had extremely significant inhibitory activity against V. harveyi (P <0. 01). [Conclusion] S. chinensis a d P. sibiricum could significantly inhibit V. harveyi and its biofilms.
- Subjects
SOLOMON'S seal; VIBRIO harveyi; BACTERICIDAL action; BIOFILMS; IN vitro studies
- Publication
Agricultural Biotechnology (2164-4993), 2016, Vol 5, Issue 4, p57
- ISSN
2164-4993
- Publication type
Article