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- Title
Influence of temperature on infection and establishment of ' Candidatus Liberibacter americanus' and ' Candidatus Liberibacter asiaticus' in citrus plants.
- Authors
Gasparoto, M. C. G.; Coletta-Filho, H. D.; Bassanezi, R. B.; Lopes, S. A.; Lourenço, S. A.; Amorim, L.
- Abstract
The objectives of this work were (i) to determine the influence of temperature on infection of citrus by ' Candidatus Liberibacter asiaticus' and ' Candidatus Liberibacter americanus', the two bacterial species associated with citrus huanglongbing (HLB) in Brazil, and (ii) to determine the influence of temperature on citrus colonization by ' Ca. L. asiaticus', which has taken over from ' Ca. L. americanus' as the predominant species in Brazil since 2008. Two experiments were carried out with graft-inoculated Valencia oranges on Rangpur lime rootstocks. Immediately after inoculation the plants were maintained for 423 days in growth chambers under the following night/day temperature conditions: 17/22, 22/27 or 27/32°C, with a dark/light photoperiod of 8/16 h. Infection and colonization of plants were determined using quantitative PCR (qPCR). ' Candidatus Liberibacter americanus' did not infect the plants maintained at 27/32°C; however, infection by ' Ca. L. asiaticus' occurred at all studied temperatures. Two months after inoculation, ' Ca. L. asiaticus' was distributed throughout the inoculated plants, with mean Ct values in the range of 30-31 for leaves and 25-28 for roots. Over time, ' Ca. L. asiaticus' reached the highest titres in mature leaves (mean Ct value = 26·7) of citrus plants maintained at 22/27°C. ' Candidatus Liberibacter asiaticus' colonization of citrus plants was negatively affected by the daily temperature regime of 27/32°C (mean Ct value in mature leaves = 33·6).
- Subjects
BRAZIL; TEMPERATURE of plants; CANDIDATUS liberibacter asiaticus; PLANT diseases; CITRUS fruits
- Publication
Plant Pathology, 2012, Vol 61, Issue 4, p658
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/j.1365-3059.2011.02569.x