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- Title
Gastrointestinal stromal tumors and KIT-positive mesenchymal cells in the omentum.
- Authors
Sakurai, Shinji; Hishima, Tunekazu; Takazawa, Yutaka; Sano, Takaaki; Nakajima, Takashi; Saito, Ken; Morinaga, Shojiroh; Fukayama, Masashi
- Abstract
Gastrointestinal stromal tumor (GIST) is currently considered to be derived from the interstitial cells of Cajal (ICC). To test the hypothesis that omental mesenchymal tumor is also a type of GIST, we evaluated the expression of specific molecules in GIST, and c-kit gene mutation in omental mesenchymal tumors, and we identified a possible counterpart of ICC in the omentum. Immunohistochemically, all of the omental mesenchymal tumors (n = 5) were positive for both KIT and CD34, and three of the five tumors were also positive for an embryonic form of smooth-muscle myosin heavy chain (SMemb). Polymerase chain reaction–single-strand conformational polymorphism analysis (PCR–SSCP) and direct sequencing revealed mutations in c-kit gene exon 11 in all five tumors. As for the ICC counterparts in the omentum, there were some KIT-positive mesenchymal cells resembling ICC at the surface of the omentum. Double fluorescence immunostaining, using anti-KIT polyclonal antibodies and monoclonal antibodies against other molecules, demonstrated that KIT-, CD34- and SMemb-positive cells were present just beneath the mesothelial cells of the omentum. These results show that omental mesenchymal tumor corresponds to GIST of the omentum, and that KIT-positive bipolar mesenchymal cells may be a counterpart of ICC in the gastrointestinal tract. Identification of a new type of KIT-positive mesenchymal cell in the omentum may lead to the discovery of a new physiological role for this organ.
- Subjects
GASTROINTESTINAL tumors; OMENTUM tumors; IMMUNOHISTOCHEMISTRY
- Publication
Pathology International, 2001, Vol 51, Issue 7, p524
- ISSN
1320-5463
- Publication type
Article
- DOI
10.1046/j.1440-1827.2001.01224.x