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- Title
Different roles of proteolipids and 70-kDa subunits of V-ATPase in growth and death of cultured human cells.
- Authors
Zhan, Hong; Yokoyama, Ken; Otani, Hajime; Tanigaki, Keiji; Shirota, Naomi; Takano, Syuichi; Ohkuma, Shoji
- Abstract
Abstract Background: The vacuolar-type proton-translocating adenosine triphosphatase (V-ATPase) plays important roles in cell growth and tumour progression. V-ATPase is composed of two distinct structures, a hydrophilic catalytic cytosolic sector (V1 ) and a hydrophobic transmembrane sector (V0 ). The V1 sector is composed of 5–8 different subunits with the structure A3 B3 C1 D1 E1 F1 G1 H1 . The V0 sector is composed of 5 different subunits with the structure 1161 381 191 166 . The over-expression of 16-kDa proteolipid subunit of V-ATPase in the perinuclear region of the human adventitial fibroblasts promotes phenotypic modulation that contributes to neointimal formation and medial thickening. A relationship between oncogenicity and the expression of the 16-kDa proteolipid has also been suggested in human pancreatic carcinoma tissue. Results: We found that the mRNA levels of the 16-kDa proteolipid but not of the 70-kDa subunit of V-ATPase in human myofibroblasts were more abundant in serum-containing medium (MF(+) cells) than serum-free medium (MF(–) cells). In HeLa cells, the levels of mRNA and protein of the 16-kDa, 21-kDa or 70-kDa were clearly suppressed when the corresponding anti-sense oligonucleotides were administered to the culture medium. The growth rate and viability (mostly due to necrosis) of HeLa cells were reduced markedly by the 16-kDa and 21-kDa anti-sense, but little by the 70-kDa anti-sense, and not at all by any sense oligonucleotides. The localization of 16-kDa/21-kDa proteolipid subunits was different from that of the 70-kDa subunit in HeLa cells. Conclusion: These results suggest that the 16-kDa and 21-kDa proteolipid subunits of the V0 sector play crucial roles in growth and death of cultured human cells. Our results may provide new...
- Subjects
EUKARYOTIC cells; ADENOSINE triphosphatase
- Publication
Genes to Cells, 2003, Vol 8, Issue 6, p501
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1046/j.1365-2443.2003.00651.x