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- Title
A deubiquitinating enzyme, UCH/CeUBP130, has an essential role in the formation of a functional microtubule-organizing centre (MTOC) during early cleavage in C. elegans.
- Authors
Lee, Jungsoo; Jee, Changhoon; Lee, Jin Il; Lee, Myon Hee; Lee, Moon Hee; Koo, Hyeon-Sook; Chung, Chin Ha; Ahnn, Joohong
- Abstract
BackgroundDeubiquitinating enzymes generate monomeric ubiquitin in protein degradation pathways and are known to be important for the early development in many organisms. ResultsRNA interference experiments targeted for a UBP homologue, UCH/CeUBP130, in C. elegans resulted in cell division defective embryos. Immunostaining localized UCH/CeUBP130 in the sperm and at the microtubule-organizing centre (MTOC) during early cleavage. Furthermore, the embryonic lethal phenotype was rescued by mating with wild-type males. ConclusionsSince it is known that the MTOC in the fertilized embryo is contributed by sperm asters in C. elegans, we suggest that UCH/CeUBP130 and ubiquitin protein degradation pathways may be involved in microtubule-based sperm aster formation. Therefore UCH/CeUBP130 is necessary for the formation of a functional MTOC in the fertilized embryo of C. elegans.
- Subjects
HYDROLASES; UBIQUITIN; PROTEIN deamination; MICROTUBULES; CAENORHABDITIS elegans; GENETICS; BIOSYNTHESIS
- Publication
Genes to Cells, 2001, Vol 6, Issue 10, p899
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1046/j.1365-2443.2001.00471.x