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- Title
Evaluation of hypoxic tissue dynamics with 18F-FMISO PET in a rat model of permanent cerebral ischemia.
- Authors
Rojas, Santiago; Herance, José Raul; Abad, Sergio; Jiménez, Xavier; Pareto, Deborah; Ruiz, Alba; Torrent, Èlia; Figueiras, Francisca P.; Popota, Foteini; Fernández-Soriano, Francisco J.; Planas, Anna M.; Gispert, Juan D.; Herance, José Raul; Jiménez, Xavier; Torrent, Èlia; Fernández-Soriano, Francisco J; Torrent, Elia
- Abstract
<bold>Purpose: </bold>[¹⁸F]Fluoromisonidazole (¹⁸F-FMISO) is a nitroimidazole derivative that has been proposed as a positron emission tomography (PET) radiotracer to detect hypoxic tissue in vivo. This compound accumulates in hypoxic but viable tissue and may be a good candidate for evaluating the ischemic penumbra. We evaluated the time course of ¹⁸F-FMISO uptake using PET in a rat model of permanent cerebral ischemia and the correlation with histological changes.<bold>Procedures: </bold>Rats (n = 14) were subjected to permanent ischemia by intraluminal occlusion of the middle cerebral artery in order to assess by PET the uptake of ¹⁸F-FMISO at various times over 24 h following ischemia. The PET results were compared to histological changes with Nissl and 2,3,5 triphenyltetrazolium chloride staining.<bold>Results: </bold>Elevated uptake of ¹⁸F-FMISO was detected in the infarcted area up to 8 h after occlusion but was no longer detected at 24 h, a time point coincident with pan necrosis of the tissue.<bold>Conclusions: </bold>Our findings suggest that salvageable tissue persists for up to 8 h in this rat model of brain ischemia. We propose ¹⁸F-FMISO PET as a tool for evaluating the ischemic penumbra after cerebral ischemia.
- Subjects
HYPOXEMIA; POSITRON emission tomography; CEREBRAL ischemia; CHLORIDES; NITROIMIDAZOLES; LABORATORY rats
- Publication
Molecular Imaging & Biology, 2011, Vol 13, Issue 3, p558
- ISSN
1536-1632
- Publication type
journal article
- DOI
10.1007/s11307-010-0371-4