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- Title
Insecticidal Activity of Crystals and Spores of Purified Bacillus thuringiensis 00-50-5 Strain against Trichoplusia ni Hubner.
- Authors
Liu Liping; Cheng Bai; Song Yingzi; Chen Yipeng; Peng Zhengqiang
- Abstract
[Objective] The paper was to study pathogenic mechanism and action mode of Bacillus thuringiensis (Bt) 00-50-5 strain against Trichoplusia ni Hubner. [Method] The crystals and spores of B. thuringiensis 00-50-5 strain were first isolated and purified, and their insecticidal activities against T. ni were compared. [Result] Either the mixture of pure crystals and pure spores of 00-50-5 strain, or only pure crystals could kill T. ni larvae under the concentration of 100 μg/mL after 48 h, and the mortality rate reached 100% ; pure spores could not kill the larvae. Determination results of median lethal concentration showed that insecticidal activity of pure crystal of Bt 00-50-5 strain (LC50 =0.32 μg/mL) was higher than the mixture of crystals and spores (LC50 =0.48) , but the insecticidal activity of pure spores was very low (LC50500.00). Therefore, the crystals were primarily responsible for causing death of larvae. [Conclusion] The paper provides theoretical basis for application of Bt 00-50-5 strain.
- Subjects
CABBAGE looper; BACILLUS thuringiensis; NOCTUIDAE; TRICHOPLUSIA; ASEXUAL reproduction; SPORES; CRYSTALS
- Publication
Plant Diseases & Pests, 2013, Vol 4, Issue 4, p5
- ISSN
2152-3932
- Publication type
Article