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- Title
"Silicon-On-Insulator"-Based Nanosensor for the Revelation of MicroRNA Markers of Autism.
- Authors
Ivanov, Yuri D.; Malsagova, Kristina A.; Goldaeva, Kristina V.; Pleshakova, Tatyana O.; Shumov, Ivan D.; Galiullin, Rafael A.; Kapustina, Svetlana I.; Iourov, Ivan Y.; Vorsanova, Svetlana G.; Ryabtsev, Stepan V.; Popov, Vladimir P.; Archakov, Alexander I.
- Abstract
MicroRNAs (miRNAs), which represent short (20 to 22 nt) non-coding RNAs, were found to play a direct role in the development of autism in children. Herein, a highly sensitive "silicon-on-insulator"-based nanosensor (SOI-NS) has been developed for the revelation of autism-associated miRNAs. This SOI-NS comprises an array of nanowire sensor structures fabricated by complementary metal–oxide–semiconductor (CMOS)-compatible technology, gas-phase etching, and nanolithography. In our experiments described herein, we demonstrate the revelation of ASD-associated miRNAs in human plasma with the SOI-NS, whose sensor elements were sensitized with oligonucleotide probes. In order to determine the concentration sensitivity of the SOI-NS, experiments on the detection of synthetic DNA analogues of autism-associated miRNAs in purified buffer were performed. The lower limit of miRNA detection attained in our experiments amounted to 10−17 M.
- Subjects
NON-coding RNA; MICRORNA; ARTIFICIAL chromosomes; AUTISM; NUCLEIC acid probes; OLIGONUCLEOTIDES
- Publication
Genes, 2022, Vol 13, Issue 2, p199
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes13020199